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2 edition of brucella - phages found in the catalog.

brucella - phages

M. J. Corbel

brucella - phages

their properties, characterisation and applications

by M. J. Corbel

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Published by Ministry of Agriculture, Fisheries and Food in Alnwick .
Written in English


Edition Notes

Originally published 1980.

StatementM.J. Corbel and E.L. Thomas.
SeriesBooklet -- 2266
ContributionsThomas, E. L., Great Britain. Ministry of Agriculture, Fisheries and Food.
ID Numbers
Open LibraryOL16672461M

Campbell, G. A., and Adams, L. G., , The long-term culture of bovine-derived macro-phages and their use in the study of intracellular proliferation of Brucella abortus. Vet. Immunol. Immunopathol. – PubMed CrossRef Google Scholar The causative agent is the facultative intracellular bacterial pathogen belonging to the genus Brucella and is of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macro phages. Infect Immun Buy this book on publisher's site;

Abstract. The genus Brucella historically has been classified in the family Brucellaceae, but it was recently removed from this family and placed with several other genera in a group of unaffiliated Gram-negative aerobes. Members of this genus are important bacteria because of the widespread and serious nature of the disease they can cause in both man and ://   Title: The Use of Phages for the Removal of Infectious Biofilms VOLUME: 9 ISSUE: 4 Author(s):J. Azeredo and I. W. Sutherland Affiliation:IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal. Keywords:Biofilms, Phages, antibiotic therapies, exopolymeric matrix, polysaccharide lyase, immune system

The current study involved detection of three virulence genes (bvfA, virB, ure) by PCR in 52 isolates of Brucella melitensis biovar 3, recovered from different animal species (28 sheep, 10 goats, 9 cattle and 5 buffaloes).Of the 52 B. melitensis strains; 48 (%) isolates carried bvfA genes, 51 (%) isolates had virB genes and 50 (%) isolates were positive for ure ://   Table of Contents This guide contains general technical information for bacterial growth, propagation, preservation, and application. Additional information on bacterial culturing can be found in Bergey’s Manual of Systematic Bacteriology, 2nd Edition, published by Springer, New York1. Getting Started with an ATCC Bacterial~/media/PDFs/Culture Guides/


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Brucella - phages by M. J. Corbel Download PDF EPUB FB2

Brucella phages have been isolated and studied for about years only, and Russian, Polish and South African workers have supplied most of the standard strains. The present author has been very active in these investigations and describes methods for isolation of Brucella phages from a large collection of Brucella.

The morphology of Brucella phages has been described and authors differ as Additional Physical Format: Online version: Parnas, Józef. Brucella phages, properties and application. Basel, Karger, (OCoLC) Document Type: Brucella-Phages,-Properties-And-Qy Adobe Acrobat Reader DCDownload Adobe Acrobat Reader DC Ebook PDF:Download free Acrobat Reader DC software the only PDF viewer that lets you read search print and interact with virtually any type of PDF ://,-Properties-And-Qypdf.

Lysis by Brucella phages is a useful tool to confirm the identity of Brucella species. Brucella biovars are differentiated by their requirement for CO 2 for growth, production of urease which varies from fast to very slow, H 2 S production, sensitivity to the dyes, basic fuchsine PARNAS et al.

1,2 obtained 14 samples of Brucella bacteriophage from old cultures of Brucella abortus and compared with these a Brucella phage originally isolated from manure in the :// The author emphasizes his belief that phages may be used more extensively for the taxonomic problems of Brucella and related bacteria.

By means of tatoles and figures he shows the phage specificity for Brucella, Francisella tularensis (sometimes classified with Brucella), Pasteurella pestis, P.

multocida and P. pseudotuberculosis. By the use of phages Tb, /XXIX, at the two doses of 1 RTD For decades, bacteriophages (phages) have been used for Brucella species identification in the diagnosis and epidemiology of brucellosis.

Traditional Brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that can take up to three weeks. In this study, we focused on the use of brucellaphages for sensitive detection of the pathogen in clinical and other Phages can exploit their bacterial hosts by lytic infection, when many viral particles are released at cell lysis, or by lysogeny, when phages integrate into the host’s :// Brucella belongs to the a2 proteobacteria, its closest relatives being predominantly plant pathogens such as Agrobacterium and Ochrobacter species, and in a more distant relationship, Bartonella la is a small Gram-negative coccobacillus that grows slowly and, in traditional culturing methods, may require specific growth media.

The recent sequencing of the complete genome of   Phages were spotted onto the surface of the plates at a titre of the order 10 9 phages/ml with either a cross test comb or a pipette both holding 10 μl of individual phage lysates.

The plates were left to dry and were inspected for lysis zones after an overnight incubation at 30°C. The spot assay was used to assess the bactericide ability of   Rev. sci. tech. Off. int. Epiz.,1 (1), Classification of the genus Brucella: the current position(*) by M.J.

CORBEL and W.J. BRIIMLEY MORGAN(**) The present system of taxonomy for the genus Brucella is based on recom­ mendations made by the Sub-Committee on Taxonomy of Brucella of the International Committee on Bacteriological Nomenclature () (1) and sub­ Brucella melitensis is a major problem in many countries Figure 2 Epididymitis (tail of epididymides) in a bull infected by B.

melitensis Figure 3 Stamp stain (modified Gimenez method) of vaginal swabs from aborted ewes Table A.3 Lytic activity of phages for smooth (S) and rough (R) Brucella species   plural of brucellaphage Definition from Wiktionary, the free dictionary Brucella spp.

are facultative intracellular pathogens that have the ability to survive and multiply in professional and non-professional phagocytes, and cause abortion in domestic animals and undulant fever in l species are recognized within the genus Brucella and this classification is mainly based on the difference in pathogenicity and in host ://   Lysis by bacteriophage is also useful for identification.

Phages that can lyse members of the genus Brucella do not lyse members of other genera and thus can be useful in generic identification.

There are several phages active or partially active upon various members of this genus. The most dependable and useful is the Tbilisi :// This is a continuation of the work of PARNAS and ZALICHTA [above] who reported on the inhibition of the activity of Brucella phages by acetone preparations of the three types of Brucella, especially by a substrate of Br.

melitensis; living suspensions of Br. melitensis are normally not sensitive to Brucella phages. The two same phages (Tb and /XV) and the indicator strain (Br.

abortus 12 A total of strains of Brucella were tested foi phage sensitivity against 22 Brucella phages. The strains of Brucella were of bovine, human and sheep origin and some were unknown; they had been classified as, Br.

abortus, intermediate, the British melitensis variant (now recognized as abortus by their metabolic-activities), ' Br, suis, and Br. :// There is some controversy about whether the typing of Brucella by the use of selected Brucella phages is valid.

In all, strains of Brucella suis have been tested against 22 phages in the routine test dilution (RTD) and at 10, RTD. The strains of Br. suis were from many countries and were in the smooth phase and gave the recognized biochemical and serological reactions of Br.

suis. The Ackermann, H. W., F. Simon, and J. Verger. A survey of Brucella phages and morphology of new isolates Intervirology 16 1–7 PubMed CrossRef Google Scholar Ackermann, M. R., N. Cheville, and B. Deyoe. Bovine ileal dome lymphoepithelial cells: Endocytosis and transport of Brucella abortus strain 19 ://?page=1.

Hanson Wade's goal is to accelerate progress within organisations and across industries. Our primary method for achieving this is by creating exclusive business conferences that gather together the world's smartest thinkers and ://.

The Brucella phages their properties characterization and applications Book. Oct ; Michael J Corbel The properties of the genus Brucella are summarized and recommendations made for the   Brucella phages.

Table (2): Brucella phages for genus/ species identification of isolated brucellae. Phage group. Propagating strain. Source. 1. Tbilisi Holger C Scholz's research works with 5, citations reads, including: A headache with surprising outcome: first case of brucellosis caused by Brucella suis biovar 1 in Germany